CHAPTER XXIV

STUDIES IN CRYPTOGAMS

The pupil who has acquired skill in the use of the compound microscope may desire to make more extended excursions into the cryptogamous orders. The following plants have been chosen as examples in various groups. Ferns are sufficiently discussed in the preceding chapter.

BACTERIA

If an infusion of ordinary hay is made in water and allowed to stand, it becomes turbid or cloudy after a few days, and a drop under the microscope will show the presence of minute oblong cells swimming in the water perhaps by means of numerous hair-like appendages, that project through the cell wall from the protoplasm within. At the surface of the dish containing the infusion the cells are non-motile and are united in long chains. Each of these cells or organisms is a _bacterium_ (plural, _bacteria_). (Fig. 135.)

Bacteria are very minute organisms,--the smallest known,--consisting either of separate oblong or spherical cells, or of chains, plates, or groups of such cells, depending on the kind. They possess a membrane-like wall which, unlike the cell walls of higher plants, contains nitrogen. The presence of a nucleus has not been definitely demonstrated. Multiplication is by the fission of the vegetative cells; but under certain conditions of drought, cold, or exhaustion of the nutrient medium, the protoplasm of the ordinary cells may become invested with a thick wall, thus forming an _endospore_ which is very resistant to extremes of environment. No sexual reproduction is known.

Bacteria are very widely distributed as parasites and saprophytes in almost all conceivable places. _Decay_ is largely caused by bacteria, accompanied in animal tissue by the liberation of foul-smelling gases. Certain species grow in the reservoirs and pipes of water supplies, rendering the water brackish and often undrinkable. Some kinds of _fermentation_ (the breaking down or decomposing of organic compounds, usually accompanied by the formation of gas) are due to these organisms. Other bacteria oxidize alcohol to _acetic acid_, and produce _lactic acid_ in milk and _butyric acid_ in butter. Bacteria live in the mouth, stomach, intestines, and on the surface of the skin of animals. Some secrete gelatinous sheaths around themselves; others secrete sulfur or iron, giving the substratum a vivid color.

Were it not for bacteria, man could not live on the earth, for not only are they agents in the process of decay, but they are concerned in certain healthful processes of plants and animals. We have learned in Chap. VIII how bacteria are related to nitrogen-gathering.

Bacteria are of economic importance not alone because of their effect on materials used by man, but also because of the _disease-producing power_ of certain species. _Pus_ is caused by a spherical form, _tetanus_ or _lock-jaw_ by a rod-shaped form, _diphtheria_ by short oblong chains, _tuberculosis_ or “_consumption_” by more slender oblong chains, and _typhoid fever_, _cholera_, and other diseases by other forms. Many _diseases of animals and plants_ are caused by bacteria. Disease-producing bacteria are said to be _pathogenic_.

The ability to grow in other nutrient substances than the natural one has greatly facilitated the study of these minute forms of life. By the use of suitable culture media and proper precautions, _pure cultures_ of a particular disease-producing bacterium may be obtained with which further experiments may be conducted.

Milk provides an excellent collecting place for bacteria coming from the air, from the coat of the cow and from the milker. Disease germs are sometimes carried in milk. If a drop of milk is spread on a culture medium (as agar), and provided with proper temperature, the bacteria will multiply, each one forming a colony visible to the naked eye. In this way, the number of bacteria originally contained in the milk may be counted.

Bacteria are disseminated in water, as the germ of typhoid fever and cholera; in milk and other fluids; in the air; and on the bodies of flies, feet of birds, and otherwise.

Bacteria are thought by many to have descended from algæ by the loss of chlorophyll and decrease in size due to the more specialized acquired saprophytic and parasitic habit.

ALGÆ

The algæ comprise most of the green floating “scum” which covers the surfaces of ponds and other quiet waters. The masses of plants are often called “frog spittle.” Others are attached to stones, pieces of wood, and other objects submerged in streams and lakes, and many are found on moist ground and on dripping rocks. Aside from these, all the plants commonly known as seaweeds belong to this category; these latter are inhabitants of salt water.

The simplest forms of algæ consist of a single spherical cell, which multiplies by repeated division or fission. Many of the forms found in fresh water are filamentous, _i.e._ the plant body consists of long threads, either simple or branched. Such a plant body is termed a _thallus_. This term applies to the vegetative body of all plants that are _not differentiated into stem and leaves_. Such plants are known as _thallophytes_ (p. 181). All algæ contain chlorophyll, and are able to assimilate carbon dioxid from the air. This distinguishes them from the fungi.

_Nostoc._--On wet rocks and damp soil dark, semitransparent irregular or spherical gelatinous masses about the size of a pea are often found. These consist of a colony of contorted filamentous algæ embedded in the jelly-like mass. The chain of cells in the filament is necklace-like. Each cell is homogeneous, without apparent nucleus, and blue-green in color, except one cell which is larger and clearer than the rest. The plant therefore belongs to the group of _blue-green algæ_. The jelly probably serves to maintain a more even moisture and to provide mechanical protection. Multiplication is wholly by the breaking up of the threads. Occasionally certain cells of the filament thicken to become _resting-spores_, but no other spore formation occurs.

[Illustration: FIG. 264.--FILAMENT OF OSCILLATORIA, showing one dead cell where the strand will break.]

_Oscillatoria._--The blue-green coatings found on damp soil and in water frequently show under the microscope the presence of filamentous algæ composed of many short homogeneous cells (Fig. 264). If watched closely, some filaments will be seen to wave back and forth slowly, showing a peculiar power of movement characteristic of this plant. Multiplication is by the breaking up of the threads. There is no true spore formation.

[Illustration: FIG. 265.--_Strand of Spirogyra_, showing the chlorophyll bands. There is a nucleus at _a_. How many cells, or parts of cells, are shown in this figure?]

SPIROGYRA.--One of the most common forms of the green algæ is spirogyra (Fig. 265). This plant often forms the greater part of the floating green mass (or “frog spittle”) on ponds. The threadlike character of the thallus can be seen with the naked eye or with a hand lens, but to study it carefully a microscope magnifying two hundred diameters or more must be used. The thread is divided into long cells by cross walls which, according to the species, are either straight or curiously folded (Fig. 266). The chlorophyll is arranged in _beautiful spiral bands_ near the wall of each cell. From the character of these bands the plant takes its name. Each cell is provided with a _nucleus_ and other _protoplasm_. The nucleus is suspended near the center of the cell (_a_, Fig. 265) by delicate strands of protoplasm radiating toward the wall and terminating at certain points in the chlorophyll band. The remainder of the protoplasm forms a thin layer lining the wall. The interior of the cell is filled with cell-sap. The protoplasm and nucleus cannot be easily seen, but if the plant is stained with a dilute alcoholic solution of eosin they become clear.

Spirogyra is propagated vegetatively by the breaking off of parts of the threads, which continue to grow as new plants. Resting-spores, which may remain dormant for a time, are formed by a process known as _conjugation_. Two threads lying side by side send out short projections, usually from all the cells of a long series (Fig. 266). The projections or processes from opposite cells grow toward each other, meet, and fuse, forming a connecting tube between the cells. The protoplasm, nucleus, and chlorophyll band of one cell now pass through this tube, and unite with the contents of the other cell. The entire mass then becomes surrounded by a thick cellulose wall, thus completing the _resting-spore_, or _zygospore_ (_z_, Fig. 266).

[Illustration: FIG. 266.--CONJUGATION OF SPIROGYRA. Ripe zygospores on the left; _a_, connecting tubes.]

[Illustration: FIG. 267.--STRAND, OR FILAMENT OF ZYGNEMA, freed from its gelatinous covering.]

_Zygnema_ is an alga closely related to spirogyra and found in similar places. Its life history is practically the same, but it differs from spirogyra in having _two star-shaped chlorophyll bodies_ (Fig. 267) in each cell, instead of a chlorophyll-bearing spiral band.

[Illustration: FIG. 268.--THREAD OF VAUCHERIA WITH OÖGONIA AND ANTHERIDIA.]

_Vaucheria_ is another alga common in shallow water and on damp soil. The thallus is much branched, but the threads are not divided by cross walls as in spirogyra. The plants are attached by means of colorless root-like organs which are much like the root hairs of the higher plants: these are _rhizoids_. The chlorophyll is in the form of _grains scattered through the thread_.

Vaucheria has a special mode of asexual reproduction by means of swimming spores or _swarm-spores_. These are formed singly in a short enlarged lateral branch known as the _sporangium_. When the sporangium bursts, the entire contents escape, forming a single large swarm-spore, which swims about by means of numerous lashes or cilia on its surface. The swarm spores are so large that they can be seen with the naked eye. After swimming about for some time they come to rest and germinate, producing a new plant.

The formation of resting-spores of vaucheria is accomplished by means of special organs, _oögonia_ (_o_, Fig. 268) and _antheridia_ (_a_, Fig. 268). Both of these are specially developed branches from the thallus. The antheridia are nearly cylindrical, and curved toward the oögonia. The upper part of an antheridium is cut off by a cross wall, and within it numerous ciliated _sperm-cells_ are formed. These escape by the ruptured apex of the antheridium. The oögonia are more enlarged than the antheridia, and have a beak-like projection turned a little to one side of the apex. They are separated from the thallus thread by a cross wall, and contain a single large green cell, the _egg-cell_. The apex of the oögonium is dissolved, and through the opening the sperm-cells enter. Fertilization is thus accomplished. After fertilization the egg-cell becomes invested with a thick wall and is thus converted into a resting-spore, the _oöspore_.

_Fucus._--These are rather large specialized algæ belonging to the group known as brown seaweeds and found attached by a disk to the rocks of the seashore just below high tide (Fig. 269). They are firm and strong to resist wave action and are so attached as to avoid being washed ashore. They are very abundant algæ. In shape the plants are long, branched, and multicellular, with either flat or terete branches. They are olive-brown. Propagation is by the breaking off of the branches. No zoöspores are produced, as in many other seaweeds; and reproduction is wholly sexual. The _antheridia_, bearing _sperm-cells_, and the _oögonia_, each bearing eight _egg-cells_, are sunken in pits or _conceptacles_. These pits are aggregated in the swollen lighter colored tips of some of the branches (_s_, _s_, Fig. 269). The egg-cells and sperm-cells escape from the pits and fertilization takes place in the water. The matured eggs, or spores, reproduce the fucus plant directly.

[Illustration: FIG. 269.--FUCUS. Fruiting branches at _s_, _s_. On the stem are two air-bladders.]

[Illustration: FIG. 270.--NITELLA.]

_Nitella._--This is a large branched and specialized fresh-water alga found in tufts attached to the bottom in shallow ponds (Fig. 270). Between the whorls of branches are long _internodes consisting of a single cylindrical cell_, which is one of the largest cells known in vegetable tissue. Under the microscope the walls of this cell are found to be lined with a layer of small stationary chloroplastids, within which layer the protoplasm, under favorable circumstances, will be found in motion, moving up one side and down the other (in rotation). Note the clear streak up the side of the cell and its relation to the moving current.

FUNGI

Some forms of fungi are familiar to every one. Mushrooms and toadstools, with their varied forms and colors, are common in fields, woods, and pastures. In every household the common molds are familiar intruders, appearing on old bread, vegetables, and even within tightly sealed fruit jars, where they form a felt-like layer dusted over with blue, yellow, or black powder. The strange occurrence of these plants long mystified people, who thought they were productions of the dead matter upon which they grew, but now we know that a mold, as any other plant, cannot originate spontaneously; it must start from something which is analogous to a seed. The “seed” in this case is a _spore_. A spore may be produced by a _vegetative process_ (growing out from the ordinary plant tissues), or it may be the result of a _fertilization process_.

_Favorable conditions for the growth of fungi._--Place a piece of bread under a moist bell jar and another in an uncovered place near by. Sow mold on each. Note the result from day to day. Moisten a third piece of bread with weak copper sulfate (blue vitriol) or mercuric chlorid solution, sow mold, cover with bell jar, note results, and explain. Expose pieces of different kinds of food in a damp atmosphere and observe the variety of organisms appearing. Fungi are saprophytes or parasites, and must be provided with organic matter on which to grow. They are usually most abundant in moist places and wet seasons.

[Illustration: FIG. 271.--MUCOR MUCEDO, showing habit.]

[Illustration: FIG. 272.--SPORES OF MUCOR, some germinating.]

_Mold._--One of these molds (_Mucor mucedo_), which is very common on all decaying fruits and vegetables, is shown in Fig. 271, somewhat magnified. When fruiting, this mold appears as a _dense mass of long white hairs_, often over an inch high, standing erect from the fruit or vegetable on which it is growing.

The life of this mucor begins with a minute rounded spore (_a_, Fig. 272), which lodges on the decaying material. When the spore germinates, it sends out a delicate thread that grows rapidly in length and forms very many branches that soon permeate every part of the substance on which the plant grows (_b_, Fig. 272). One of these threads is termed a _hypha_. All the threads together form the _mycelium_ of the fungus. The mycelium disorganizes the material in which it grows, and thus the mucor plant (Fig. 271) is nourished. It corresponds physiologically to the roots and stems of other plants.

When the mycelium is about two days old, it begins to form the long fruiting stalks which we first noticed. To study them, use a compound microscope magnifying about two hundred diameters. One of the stalks, magnified, is shown in _a_, Fig. 274. It consists of a rounded head, the _sporangium, sp_, supported on a long, delicate stalk, the _sporangiophore_. The stalk is separated from the sporangium by a wall which is formed at the base of the sporangium. This wall, however, does not extend straight across the thread, but it arches up into the sporangium like an inverted pear. It is known as the _columella, c_. When the sporangium is placed in water, the wall immediately dissolves and allows hundreds of spores, which were formed in the cavity within the sporangium, to escape, _b_. All that is left of the fruit is the stalk, with the pear-shaped columella at its summit, _c_. The spores that have been set free by the breaking of the sporangium wall are now scattered by the wind and other agents. Those that lodge in favorable places begin to grow immediately and reproduce the fungus. The others soon perish.

[Illustration: FIG. 273.--MUCOR, showing formation of zygospore on the right; germinating zygospore on the left.]

[Illustration: FIG. 274.--MUCOR.

_a_, sporangium; _b_, sporangium bursting; _c_, columella.]

The mucor may continue to reproduce itself in this way indefinitely, but these spores are very delicate and usually die if they do not fall on favorable ground, so that the fungus is provided with another means of carrying itself over unfavorable seasons, as winter. This is accomplished by means of curious _thick-walled resting-spores_ or _zygospores_. The zygospores are formed on the mycelium buried within the substance on which the plant grows. They originate in the following way: Two threads that lie near together send out short branches, which grow toward each other and finally meet (Fig. 273). The walls at the ends, _a_, then disappear, allowing the contents to flow together. At the same time, however, two other walls are formed at points farther back, _b_, _b_, separating the short section, _c_, from the remainder of the thread. This section now increases in size and becomes covered with a thick, dark brown wall ornamented with thickened tubercles. The zygospore is now mature and, after a period of rest, it germinates, either producing a sporangium directly or growing out as mycelium.

The zygospores of the mucors form one of the most interesting and instructive objects among the lower plants. They are, however, very difficult to obtain. One of the mucors (_Sporodinia grandis_) may be frequently found in summer growing on toadstools. This plant usually produces zygospores that are formed on the aërial mycelium. The zygospores are large enough to be recognized with a hand lens. The material may be dried and kept for winter study, or the zygospores may be prepared for permanent microscopic mounts in the ordinary way.

[Illustration: FIG. 275.--YEAST PLANTS.]

_Yeast._--This is a very much reduced and simple fungus, consisting normally of isolated spherical or elliptical cells (Fig. 275) containing abundant protoplasm and probably a nucleus, although the latter is not easily observed. It propagates rapidly by _budding_, which consists of the gradual extrusion of a wart-like swelling that is sooner or later cut off at the base by constriction, thus forming a separate organism. Although simple in structure, the yeast is found to be closely related to some of the higher groups of fungi as shown by the method of spore formation. When grown on special substances like potato or carrot, the contents of the cell may _form spores inside of the sac-like mother cell_, thus resembling the sac-fungi to which blue mold and mildews belong. The yeast plant is remarkable on account of its power to induce alcoholic fermentation in the media in which it grows.

There are many kinds of yeasts. One of them is found in the common _yeast cakes_. In the process of manufacture of these cakes, the yeast cells grow to a certain stage, and the material is then dried and fashioned into small cakes, each cake containing great numbers of the yeast cells. When the yeast cake is added to dough, and proper conditions of warmth and moisture are provided, the yeast grows rapidly and breaks up the sugar of the dough into carbon dioxid and alcohol. This is _fermentation_. The gases escape and puff up the dough, causing the _bread to rise_. In this loosened condition the dough is baked; if it is not baked quickly enough, _the bread_ “_falls_.” Shake up a bit of yeast cake in slightly sweetened water: the water soon becomes cloudy from the growing yeasts.

_Parasitic fungi._--Most of the molds are saprophytes. Many other fungi are parasitic on living plants and animals (Fig. 285). Some of them have complicated life histories, undergoing many changes before the original spore is again produced. The _willow mildew_ and the common _rust of wheat_ will serve to illustrate the habits of parasitic fungi.

The _willow mildew (Uncinula salicis)_.--This is one of the sac fungi. It forms white downy patches on the leaves of willows (Fig. 276). These patches consist of numerous interwoven threads that may be recognized under the microscope as the mycelium of the fungus. The mycelium in this case lives on the surface of the leaf and nourishes itself by sending short branches into the cells of the leaf to absorb food materials from them.

[Illustration: FIG. 276.--COLONIES OF WILLOW MILDEW.]

[Illustration: FIG. 277.--SUMMER-SPORES OF WILLOW MILDEW.]

[Illustration: FIG. 278.--PERITHECIUM OF WILLOW MILDEW.]

[Illustration: FIG. 279.--SECTION THROUGH PERITHECIUM OF WILLOW MILDEW.]

Numerous _summer-spores_ are formed of short, erect branches all over the white surface. One of these branches is shown in Fig. 277. When it has grown to a certain length, the upper part begins to segment or divide into spores which fall and are scattered by the wind. Those falling on other willows reproduce the fungus there. This process continues all summer, but in the later part of the season provision is made to maintain the mildew through the winter. If some of the white patches are closely examined in July or August, a number of little black bodies will be seen among the threads. These little bodies are called _perithecia_, shown in Fig. 278. To the naked eye they appear as minute specks, but when seen under a magnification of 200 diameters they present a very interesting appearance. They are hollow spherical bodies decorated around the outside with a fringe of crook-like hairs. The _resting-spores_ of the willow mildew are produced in sacs or _asci_ inclosed within the leathery perithecia. Figure 279 shows a cross-section of a perithecium with the asci arising from the bottom. The spores remain securely packed in the perithecia. They do not ripen in the autumn, but fall to the ground with the leaf, and there remain securely protected among the dead foliage. The following spring they mature and are liberated by the decay of the perithecia. They are then ready to attack the unfolding leaves of the willow and repeat the work of the summer before.

[Illustration: FIG. 280.--SORI CONTAINING TELEUTOSPORES OF WHEAT RUST.]

_The wheat rust._--The development of some of the rusts, as the common _wheat rust (Puccinia graminis)_, is even more interesting and complicated than that of the mildews. Wheat rust is also a true parasite, affecting wheat and a few other grasses. The mycelium here cannot be seen by the unaided eye, for it consists of threads which are present within the host plant, mostly in the intercellular spaces. These threads also send short branches, or _haustoria_ (Fig. 132), into the neighboring cells to absorb nutriment.

[Illustration: FIG. 281.--TELEUTOSPORE OF WHEAT RUST.]

The _resting-spores_ of wheat rust are produced in late summer, when they may be found in black lines breaking through the epidermis of the wheat stalk (black-rust stage). They are formed in masses, called _sori_ (Fig. 280), from the ends of numerous crowded mycelial strands just beneath the epidermis of the host. The individual spores are very small and can be well studied only with a microscope of high power (× about 400). They are brown two-celled bodies with a thick wall (Fig. 281). Since they are the resting or winter-spores, they are termed _teleutospores_ (“completed spores”). Usually they do not fall, but remain in the sori during winter. The following spring each cell of the teleutospore puts forth a rather stout thread, which does not grow more than several times the length of the spore and terminates in a blunt extremity. This germ tube, _promycelium_, now becomes divided into four cells by cross walls, which are formed from the top downwards. Each cell gives rise to a short, pointed branch which, in the course of a few hours, forms at its summit a single spore called a _sporidium_. This in turn germinates and produces a mycelium. In Fig. 282 a germinating teleutospore is drawn to show the promycelium, _p_, divided into four cells, each producing a short branch with a little _sporidium, s_.

[Illustration: FIG. 282.--GERMINATING TELEUTOSPORE OF WHEAT RUST.]

[Illustration: FIG. 283.--LEAF OF BARBERRY WITH CLUSTER-CUPS.]

[Illustration: FIG. 284.--SECTION THROUGH A CLUSTER-CUP ON BARBERRY LEAF.]

A most remarkable circumstance in the life history of the wheat rust is the fact that the mycelium produced by the sporidium _can live only in barberry leaves_, and it follows that if no barberry bushes are in the neighborhood the sporidia finally perish. Those which happen to lodge on a barberry bush germinate immediately, producing a mycelium that enters the barberry leaf and grows within its tissues. Very soon the fungus produces a new kind of spores on the barberry leaves. These are called _æcidiospores_. They are formed in long chains in little fringed cups, or _æcidia_, which appear in groups on the lower side of the leaf (Fig. 283). These orange or yellow æcidia are termed _cluster-cups_. In Fig. 284 is shown a cross-section of one of the cups, outlining the long chains of spores, and the mycelium in the tissues.

The æcidiospores are formed in the spring, and after they have been set free, some of them lodge on wheat or other grasses, where they germinate immediately. The germ-tube enters the leaf through a stomate, whence it spreads among the cells of the wheat plant. In summer one-celled reddish uredospores (“blight spores,” red-rust stage) are produced in a manner similar to the teleutospores. These are capable of germinating immediately, and serve to disseminate the fungus during the summer on other wheat plants or grasses. Late in the season, teleutospores are again produced, completing the life cycle of the plant.

[Illustration: FIG. 285.--HOW A PARASITIC FUNGUS WORKS. Anthracnose on a bean pod entering the bean beneath. (Whetzel.)]

Many rusts besides _Puccinia graminis_ produce different spore forms on different plants. The phenomenon is called _heterœcism_, and was first shown to exist in the wheat rust. Curiously enough, the peasants of Europe had observed and asserted that barberry bushes cause wheat to blight long before science explained the relation between the cluster-cups on barberry and the rust on wheat. The true relation was actually demonstrated, as has since been done for many other rusts on their respective hosts, by sowing the æcidiospores on healthy wheat plants and thus producing the rust. The _cedar apple_ is another rust, producing the curious swellings often found on the branches of red cedar trees. In the spring the teleutospores ooze out from the “apple” in brownish yellow masses. It has been found that these attack various fruit trees, producing æcidia on their leaves. Fig. 285 explains how a parasitic fungus works.

_Puffballs_, _mushrooms_, _toadstools_, _and shelf fungi_.--These represent what are called the _higher fungi_, because of the size and complexity of the plant body as well as from the fact that they seem to stand at the end of one line of evolution. The mycelial threads grow together in extensive strands in rotten wood or in the soil, and send out large complex growths of mycelium in connection with which the spores are borne. These aërial parts are the only ones we ordinarily see, and which constitute the “mushroom” part (Fig. 131). Only asexual spores (_basidiospores_) are produced, and on short stalks (_basidia_) (Fig. 286). In the puffballs the spores are inclosed and constitute a large part of the “smoke.” In the mushrooms and toadstools they are borne on _gills_, and in the shelf fungi (Fig. 134) on the walls of minute pores of the underside. The mycelium of these shelf fungi frequently lives and grows for a long time concealed in the substratum before the visible fruit bodies are sent out. Practically all timber decay is caused by such growth, and the damage is largely done before the fruiting bodies appear. For other accounts of mushrooms, see Chap. XIV.

[Illustration: FIG. 286.--PART OF GILL OF THE CULTIVATED MUSHROOM.

_tr_, trama tissue; _sh_, hymenium; _b_, basidium; _st_, sterigma; _sp_, spore. (Atkinson.)]

LICHENS

Lichens are so common everywhere that the attention of the student is sure to be drawn to them. They grow on rocks, trunks of trees (Fig. 287), old fences, and on the earth. They are thin, usually gray ragged objects, apparently lifeless. Their study is too difficult for beginners, but a few words of explanation may be useful.

[Illustration: FIG. 287.--LICHEN ON AN OAK TRUNK. (A species of _Physcia_.)]

Lichens were formerly supposed to be a distinct or separate division of plants. They are now known to be organisms, each species of which is a constant association of a fungus and an alga. The thallus is ordinarily made up of fungous mycelium or tissue within which the imprisoned alga is definitely distributed. The result is a growth unlike either component. This association of alga and fungus is usually spoken of as _symbiosis_, or mutually helpful growth, the alga furnishing some things, the fungus others, and both together being able to accomplish work that neither could do independently. By others this union is considered to be a mild form of parasitism, in which the fungus profits at the expense of the alga. As favorable to this view, the facts are cited that each component is able to grow independently, and that under such conditions the algal cells seem to thrive better than when imprisoned by the fungus.

Lichens propagate by means of _soredia_, which are tiny parts separated from the body of the thallus, and consisting of one or more algal cells overgrown with fungus threads. These are readily observed in many lichens. They also produce spores, usually ascospores, which are always the product of the fungus element, and which reproduce the lichen by germinating in the presence of algal cells, to which the hyphæ immediately cling.

Lichens are found in the most inhospitable places, and, by means of acids which they secrete, they attack and slowly disintegrate even the hardest rocks. By making thin sections of the thallus with a sharp razor and examining under the compound microscope, it is easy to distinguish the two components in many lichens.

LIVERWORTS

The liverworts are peculiar flat green plants usually found on wet cliffs and in other moist, shady places. They frequently occur in greenhouses where the soil is kept constantly wet. One of the commonest liverworts is _Marchantia polymorpha_, two plants of which are shown in Figs. 288, 289. The plant consists of a ribbon-like thallus that creeps along the ground, becoming repeatedly forked as it grows. The end of each branch is always conspicuously notched. There is a prominent midrib extending along the center of each branch of the thallus. On the under side of the thallus, especially along the midrib, there are numerous rhizoids which serve the purpose of roots, absorbing nourishment from the earth and holding the plant in its place. The upper surface of the thallus is divided into minute rhombic areas that can be seen with the naked eye. Each of these areas is perforated by a small breathing pore or stomate that leads into a cavity just beneath the epidermis. This space is surrounded by chlorophyll-bearing cells, some of which stand in rows from the bottom of the cavity (Fig. 290). The delicate assimilating tissue is thus brought in close communication with the outer air through the pore in the thick, protecting epidermis.

[Illustration: FIG. 288.

FIG. 289.

PLANTS OF MARCHANTIA.]

[Illustration: FIG. 290.--SECTION OF THALLUS OF MARCHANTIA. Stomate at _a_.]

At various points on the midrib are little cups containing small green bodies. These bodies are buds or _gemmæ_ which are outgrowths from the cells at the bottom of the cup. They become loosened and are then dispersed by the rain to other places, where they take root and grow into new plants.

The most striking organs on the thallus of marchantia are the peculiar stalked bodies shown in Figs. 288, 289. These are termed archegoniophores and _antheridiophores_ or _receptacles_. Their structure and function are very interesting, but their parts are so minute that they can be studied only with the aid of a microscope magnifying from 100 to 400 times. Enlarged drawings will guide the pupil.

[Illustration: FIG. 291.--SECTION THROUGH ANTHERIDIOPHORE OF MARCHANTIA, showing antheridia. One antheridium more magnified.]

The _antheridiophores_ are fleshy, lobed disks borne on short stalks (Fig. 291). The upper surface of the disk shows openings scarcely visible to the naked eye. However, a section of the disk, such as is drawn in Fig. 291, shows that the pores lead into oblong cavities in the receptacle. From the base of each cavity there arises a thick, club-shaped body, the _antheridium_. Within the antheridium are formed many sperm-cells which are capable of swimming about in water by means of long lashes or cilia attached to them. When the antheridium is mature, it bursts and allows the ciliated sperm cells to escape.

The _archegoniophores_ are also elevated on stalks (Fig. 289). Instead of a simple disk, the receptacle consists of nine or more finger-like rays. Along the under side of the rays, between delicately fringed curtains, peculiar flask-like bodies, or _archegonia_, are situated. The archegonia are not visible to the naked eye. They can be studied only with the microscope (× about 400). One of them much magnified is represented in Fig. 292. Its principal parts are the long _neck, a_, and the rounded _venter, b_, inclosing a large free cell--the egg-cell.

[Illustration: FIG. 292.--ARCHEGONIUM OF MARCHANTIA.]

[Illustration: FIG. 293.--ARCHEGONIOPHORE, WITH SPOROGONIA, OF MARCHANTIA.]

We have seen that the antheridium at maturity discharges its sperm-cells. These swim about in the water provided by the dew and rain. Some of them finally find their way to the archegonia and egg-cells, the latter being fertilized, as pollen fertilizes the ovules of higher plants.

After fertilization the egg-cell develops into the spore capsule or _sporogonium_. The mature spore capsules may be seen in Fig. 293. They consist of an oval spore-case on a short stalk, the base of which is imbedded in the tissue of the receptacle, from which it derives the necessary nourishment for the development of the sporogonium. At maturity the sporogonium is ruptured at the apex, setting free the spherical spores together with numerous filaments having spirally thickened walls (Fig. 294). These filaments are called _elaters_. When drying, they exhibit rapid movements by means of which the spores are scattered. The spores germinate and again produce the thallus of marchantia.

[Illustration: FIG. 294.--SPORES AND ELATERS OF MARCHANTIA.]

[Illustration: FIG. 295.--POLYTRICHUM COMMUNE. _f_,_f_, fertile plants, one on the left in fruit, _m_, antheridial plant.]

[Illustration: FIG. 296.--SECTION OF LEAF OF POLYTRICHUM COMMUNE.]

MOSSES (Bryophyta)

If we have followed carefully the development of marchantia, the study of one of the mosses will be comparatively easy. The mosses are more familiar plants than the liverworts. They grow on trees, stones, and on the soil both in wet and dry places. One of the common larger mosses, known as _Polytrichum commune_, may serve as an example, Fig. 295. This plant grows on rather dry knolls, mostly in the borders of open woods, where it forms large beds. In dry weather these beds have a reddish brown appearance, but when moist they form beautiful green cushions. This color is due, in the first instance, to the color of the old stems and leaves, and, in the second instance, to the peculiar action of the green living leaves under the influence of changing moisture-conditions. The inner or upper surface of the leaf is covered with thin, longitudinal ridges of delicate cells which contain chlorophyll. These cells are shown in cross-section in Fig. 296, as dots or granules. All the other tissue of the leaf consists of thick-walled, corky cells which do not allow moisture to penetrate. When the air is moist the green leaves spread out, exposing the chlorophyll cells to the air, but in dry weather the margins of the leaves roll inward, and the leaves fold closely against the stem, thus protecting the delicate assimilating tissue.

The _antheridia_ and _archegonia_ of polytrichum are borne in groups at the ends of the branches on different plants (many mosses bear both organs on the same branch). They are surrounded by involucres of characteristic leaves termed _perichætia_ or _perichætal leaves_. Multicellular hairs known as _paraphyses_ are scattered among the archegonia and antheridia. The involucres with the organs borne within them are called _receptacles_, or, less appropriately, “moss flowers.” As in marchantia, the organs are very minute and must be highly magnified to be studied.

[Illustration: FIG. 297.--SECTION THROUGH A RECEPTACLE OF POLYTRICHUM COMMUNE, showing paraphyses and antheridia.]

The antheridia are borne in broad cup-like receptacles on the antheridial plants (Fig. 297). They are much like the antheridia of marchantia, but they stand free among the paraphyses and are not sunk in cavities. At maturity they burst and allow the sperm cells or _spermatozoids_ to escape. In polytrichum, when the receptacles have fulfilled their function, the stem continues to grow from the center of the cup (_m_, Fig. 295). The archegonia are borne in other receptacles on different plants. They are like the archegonia of marchantia except that they stand erect on the end of the branch.

The _sporogonium_ which develops from the fertilized egg is shown in _a_, _b_, Fig. 295. It consists of a long, brown stalk bearing the spore-case at its summit. The base of the stalk is imbedded in the end of the moss stem by which it is nourished. The capsule is entirely inclosed by a hairy cap, the _calyptra, b_. The calyptra is really the remnant of the archegonium, which, for a time, increases in size to accommodate and protect the young growing capsule. It is finally torn loose and carried up on the spore-case. The mouth of the capsule is closed by a circular lid, the _operculum_, having a conical projection at the center.

The operculum soon drops, or it may be removed, displaying a fringe of sixty-four teeth guarding the mouth of the capsule. This ring of teeth is known as the _peristome_. In most mosses the teeth exhibit peculiar hygroscopic movements; _i.e._ when moist they bend outwards, and upon drying curve in toward the mouth of the capsule. This motion, it will be seen, serves to disperse the spores gradually over a long period of time.

Not the entire capsule is filled with spores. There are no elaters, but the center of the capsule is occupied by a columnar strand of tissue, the _columella_, which expands at the mouth into a thin, membranous disk, closing the entire mouth of the capsule except the narrow annular chink guarded by the teeth. In this moss the points of the teeth are attached to the margin of the membrane, allowing the spores to sift out through the spaces between them.

When the spores germinate they form a green, branched thread, the _protonema_. This gives rise directly to moss plants, which appear as little buds on the thread. When the moss plants have sent their little rhizoids into the earth, the protonema dies, for it is no longer necessary for the support of the little plants, and the moss plants grow independently.

_Funaria_ is a moss very common on damp, open soil. It forms green patches of small fine leaves from which arise long brown stalks terminated by curved capsules (Fig. 298). The structure is similar to that of polytrichum, except the absence of plates on the under side of the leaves, the continuous growth of the stem, the curved capsule, double peristome, monœcious rather than diœcious receptacles, and nearly glabrous unsymmetrical calyptra.

[Illustration: FIG. 298.--FUNARIA HYGROSCOPICA.]

EQUISETUMS, OR HORSETAILS (Pteridophyta)

There are about twenty-five species of equisetum, constituting the only genus of the unique family _Equisetaceæ_. Among these _E. arvense_ (Fig. 299) is common on clayey and sandy soils.

In this species the work of nutrition and that of spore production are performed by separate shoots from an underground rhizome. The fertile branches appear early in spring. The stem, which is 3 to 6 inches high, consists of a number of cylindrical, furrowed internodes, each sheathed at the base by a circle of scale leaves. The shoots are of a pale yellow color. They contain no chlorophyll, and are nourished by the food stored in the rhizome (Fig. 299).

The spores are formed on specially developed fertile leaves or _sporophylls_ which are collected into a spike or cone at the end of the stalk (_a_, Fig. 299). A single sporophyll is shown at _b_. It consists of a short stalk expanded into a broad, mushroom-like head. Several large _sporangia_ are borne on its under side. The spores formed in the sporangia are very interesting and beautiful objects when examined under the microscope (× about 200). They are spherical, green bodies, each surrounded by two spiral bands attached to the spore at their intersection, s. These bands exhibit hygroscopic movements by means of which the spores become entangled, and are held together. This is of advantage to the plant, as we shall see. All the spores are alike, but some of the _prothallia_ grow to a greater size than the others. The large prothallia produce only archegonia while the smaller ones produce _antheridia_. Both of these organs are much like those of the ferns, and fertilization is accomplished in the same way. Since the prothallia are usually diœcious, the special advantage of the spiral bands, holding the spores together so that both kinds of prothallia may be in close proximity, will be easily understood. As in the fern, the fertilized egg-cell develops into an equisetum plant.

[Illustration: FIG. 299.--EQUISETUM ARVENSE.

_st_, sterile shoot; _f_, fertile shoot showing the spike at _a_; _b_, sporophyll, with sporangia; _s_, spore.]

The sterile shoots (_st_, Fig. 299) appear much later in the season. They give rise to repeated whorls of angular or furrowed branches. The leaves are very much reduced scales, situated at the internodes. The stems are provided with chlorophyll and act as assimilating tissue, nourishing the rhizome and the fertile shoots. Nutriment is also stored in special tubers developed on the rhizome.

Other species of equisetum have only one kind of shoot--a tall, hard, leafless, green shoot with the spike at its summit. Equisetum stems are full of silex, and they are sometimes used for scouring floors and utensils; hence the common name “scouring rush.”

ISOËTES (Pteridophyta)

_Isoëtes_ or quillwort is usually found in water or damp soil on the edges of ponds and lakes. The general habit of the plant is seen in Fig. 300, _a_. It consists of a short, perennial stem bearing numerous erect, quill-like leaves with broad sheathing bases. The plants are commonly mistaken for young grasses.

[Illustration: FIG. 300.--ISOËTES, showing habit of plant at _a_; _b_, base of leaf, showing sporangium, velum, and ligule.]

Isoëtes bears two kinds of spores, large roughened ones, the _macrospores_, and small ones or _microspores_. Both kinds are formed in _sporangia_ borne in an excavation in the expanded base of the leaf. The macrospores are formed on the outer and the microspores on the inner leaves. A sporangium in the base of a leaf is shown at _b_. It is partially covered by a thin membrane, the _velum_. The minute triangular appendage at the upper end of the sporangium is called the _ligule_.

The spores are liberated by the decay of the sporangia. They form rudimentary prothallia of two kinds. The microspores produce prothallia with _antheridia_, while the macrospores produce prothallia with _archegonia_. Fertilization takes place as in the mosses or liverworts, and the fertilized egg-cell, by continued growth, gives rise again to the isoëtes plant.

CLUB-MOSSES (Pteridophyta)

The club-mosses are low trailing plants of moss-like looks and habit, although more closely allied to ferns than to true mosses. Except one genus in Florida, all our club mosses belong to the genus _Lycopodium_. They grow mostly in woods, having 1-nerved evergreen leaves arranged in four or more ranks. Some of them make long strands, as the ground pine, and are much used for Christmas decorations. The spores are all _of one kind_ or form, borne in _1-celled sporangia_ that open on the margin into two valves. The sporangia are borne in some species (Fig. 301) as small yellow bodies in the axils of the ordinary leaves near the tip of the shoot; in other species (Fig. 302) they are borne in the axils of small scales that form a catkin-like spike. The spores are very numerous, and they contain an oil that makes them inflammable. About 100 species of lycopodium are known. The plants grown by florists under the name of lycopodium are of the genus _Selaginella_, more closely allied to isoëtes, bearing two kinds of spores (microspores and macrospores).

[Illustration: FIG. 301.--A LYCOPODIUM WITH SPORANGIA IN THE AXILS OF THE FOLIAGE LEAVES. (_Lycopodium lucidulum_).]

[Illustration: FIG. 302.--A CLUB-MOSS (_Lycopodium complanatum_).]

ANIMAL BIOLOGY